Several tests have been developed for detecting the genetic material of the SARS-CoV-2 coronavirus that causes COVID-19, but these generally only allow detection of the virus during acute infection. Antibody, or serological, tests are urgently needed to determine the real rate of infection, to understand individuals’ antibody responses to the virus, and to identify people who are potentially immune to re-infection. As described in a paper published in Current Protocols in Microbiology, investigators have now developed such a test.
The published article provides a step-by-step protocol with enough detail for other researchers to replicate in their laboratories. The protocol involves expressing and purifying segments of a key SARS-CoV-2 protein that are then used in blood tests for the presence of antiviral antibodies.
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